MCQ of unit 2 of Microbiology 3rd semester B.Pharmacy
1. The stains which differentiate two groups of bacteria in a mixture are known as:
- (a) Negative stain
- (b) Simple stain
- (c) Differential stain
- (d) Indirect stain
Answer:- (c) Differential stain
2. The purpose of simple staining is to demonstrate:
- (a) Cell size
- (b) Cell shape
- (c) Arrangement of bacterial cells
- (d) All the above
Answer:- (d) All the above
3. Mordant used in Gram staining is:
- (a) Lugol’s iodine
- (b) Acetone
- (c) Peptone
- (d) Crystal violet
Answer:- (a) Lugol’s iodine
4. Counterstain used in Gram staining is:
- (a) Lugol’s iodine
- (b) Safranin
- (c) Peptone
- (d) Crystal violet
Answer:- (b) Safranin
5. Acid-fast staining is also known as:
- (a) Negative staining
- (b) AFB staining
- (c) Ziehl-Neelsen staining
- (d) Both b & c
Answer:- (d) Both b & c
6. IMVIC test is used to differentiate the gram negative enteric bacteria belonging to family:
- (a) Enterobacteriaceae
- (b) Chlamydaicae
- (c) Mycobacteriaceae
- (d) None of the above
Answer:- (a) Enterobacteriaceae
7. Citrate utilization test is usually performed on:
- (a) Chocolate agar
- (b) Simmon’s citrate agar
- (c) Nutrient agar
- (d) MacConkey agar
Answer:- (b) Simmon’s citrate agar
8. The process of killing microorganisms including spores is:
- (a) Disinfection
- (b) Sanitization
- (c) Sterilization
- (d) Filtration
Answer:- (c) Sterilization
9. Sanitization is mostly used in:
- (a) Hospitals
- (b) Research laboratories
- (c) Food processing & catering units
- (d) All the above
Answer:- (c) Food processing & catering units
10. Paraffin oil can be sterilized by:
- (a) Hot air oven
- (b) Autoclaving
- (c) Filtration
- (d) Disinfection
Answer:- (a) Hot air oven
11. The flash & holder methods are used in the process of:
- (a) Immunization
- (b) Disinfection
- (c) Pasteurization
- (d) Both a & b
Answer:- (c) Pasteurization
12. The process of sterilization by intermittent heating for 3 successive days is known as:
- (a) Inspissation
- (b) Tyndallization
- (c) Pasteurization
- (d) Incineration
Answer:- (b) Tyndallization
13. Best autoclaving is achieved at:
- (a) 121°C for 15 minutes
- (b) 120°C for 1 hour
- (c) 110°C for 2 hours
- (d) 100°C for 3 hours
Answer:- (a) 121°C for 15 minutes
14. Burning to ashes is referred to as:
- (a) Inertization
- (b) Sanitization
- (c) Incineration
- (d) Disinfection
Answer:- (c) Incineration
15. By inspissation repeated for three consecutive days, some degree of sterilization is achieved which is known as:
- (a) Fractional sterilization
- (b) Sanitization
- (c) Incineration
- (d) Disinfection
Answer:- (a) Fractional sterilization
16. Filter which is disposable of single-use discs made up of asbestos (magnesium trisilicate) is:
- (a) Sintered glass filter
- (b) Seitz filter
- (c) Membrane filter
- (d) Berkefield filter
Answer:- (b) Seitz filter
17. Sintered glass filter includes:
- (a) Fritted glass filter
- (b) Morton filters
- (c) Both a & b
- (d) Berkefield filter
Answer:- (c) Both a & b
18. Filters of different grades of porosity and used widely for purification of water for industrial and drinking purposes are:
- (a) Sintered glass filter
- (b) Seitz filter
- (c) Membrane filter
- (d) Berkefield filter
Answer:- (d) Berkefield filter
19. Sterilization by ionizing radiation is also known as:
- (a) Cold sterilization
- (b) Thermosterilization
- (c) Electrosterilization
- (d) None of the above
Answer:- (a) Cold sterilization
20. Which of the following chemicals in gaseous form can be used disinfection and sterilization of enclosed area such as operation theatres, hospital rooms, aseptic area and microbiology laboratories?
- (a) Glutaraldehdyde
- (b) Formaldehyde
- (c) Ethylene oxide
- (d) None of the above
Answer:- (b) Formaldehyde
21. Sterilant gas used for sterilization of heat sensitive plastic disposable items is:
- (a) Glutaraldehdyde
- (b) Formaldehyde
- (c) Ethylene oxide
- (d) None of the above
Answer:- (c) Ethylene oxide
22. Which of the following are chemical indicators of sterilization?
- (a) Browne’s tube
- (b) Witness tube
- (c) Royce sachet
- (d) All the above
Answer:- (d) All the above