MCQ / Multiple Choice Question B.Pharm 3rd semester microbiology

MCQ with answer of unit-1 of Microbiology 3rd semester B.Pharmacy.

1. Microorganisms which grow in absence of oxygen are called:

(a) Aerobic

(b) Microscopic

(c) Anaerobic

 (d) None of the above

Answer:-  (c) Anaerobic

2. The process of sterilization is used in dairy industry is:

(a) Pasteurization

(b) Tyndallization

(c) Fermentation

(d) Segregation

Answer:- (a) Pasteurization

3. The act of weakening the pathogenic property of any pathogenic microorganism is known as:

(a) Vaccination

(b) Tyndallization

(c) Sterilization

(d) Attenuation

Answer:- (d) Attenuation

4. In the process of autoclaving:

(a) Only vegetative cell gets killed

(b) Spore & vegetative cell gets killed

(c) Spores get killed only

(d) Nucleus of the cell gets killed

Answer:- (b) Spore & vegetative cell gets killed

5. The anaerobic conversion of sugar into alcohol by microorganisms is known as:

(a) Sterilization

(b) Pasteurization

(c) Fermentation

(d) Sanitization

Answer:- (c) Fermentation

6. The term vaccine was given by:

(a) R. Koch

(b) E. Jenner

(c) L. Pasteur

 (d) Flaming

Answer:- (b) E. Jenner

7. Swan neck experiment to disapprove spontaneous generation theory was performed by:

(a) L. Pasteur

(b) R. Koch

(c) Loeffler

(d) Needham

Answer:- (a) L. Pasteur

8. Agar as a solidifying agent was first time used by:

(a)  L.Pasteur

(b)  Fleming

(c)  R.koch

(d) Leeuwenhoek

Answer:- (c)  R.koch

9. Penicillin was discovered by:

(a) R. Koch

(b) Landsteiner

(c) Fleming

(d) E. Jenner

Answer:- (c) Fleming

10. Paul Erich coined the term:

(a) Physiotherapy

(b) Hydrotherapy

(c) Chemotherapy

(d) All of the above

Answer:- (c) Chemotherapy

11. The fourth postulate of Koch postulate was appended by:

(a) E. Jenner

(b) Ruska

(c) L. Pasteur

(d) E.F. Smith

Answer:- (d) E.F. Smith

12. Which of the following scientist is known as Father of Antiseptic surgery?

(a) Reddi

(b) L. Pasteur

(c) Joseph Lister

(d) Leuwenhoek

Answer:- (c) Joseph Lister

13. Microorganism have diameter usually:

(a) Greater than 1mm

(b) Less than 0.1mm

(c) Less than 1mm

(d) Greater than 0.1mm

Answer:- (b) Less than 0.1mm

14. The theory which says microorganisms evolved on earth spontaneously is:

(a) Big bang theory

(b) Natural selection theory

(c) Theory of spontaneous generation

(d) Theory of probability

Answer:- (c) Theory of spontaneous generation

15. The term used for microorganisms by Leuwenhoek is:

(a) Germ

(b) Dierken

(d) Both b & c

(c) Microbe

Answer:- (b) Dierken

16. The process of intermittent boiling/steaming at 100°C in three successive days for 30 minutes is known as:

(a) Sterilization

(b) Pasteurization

(c) Tyndallization

(d) Disinfection

Answer:- (c) Tyndallization

17. Which of the following scientist showed that malaria is transmitted by the Anopheles mosquito?

(a) Ronald Ross

(b) Rous

(c) Yersin

(d) Fraenkel

Answer:- (a) Ronald Ross

18. Study of fungi is known as:

(a) Mycology

(b) parasitology

(c) Virology

(d) Bacteriology

Answer:- (a) Mycology

19. The microscope with focusing device was first time discovered by:

(a) Ruska

(b) Bacon

(c) Faber

(d) Galileo Galilei

Answer:- (d) Galileo Galilei

20. Which of the following scientist is known as father of modern microbiology?

(a) Ruska

(b) Louis Pasteur

(c) Loffler

(d) Hansen

Answer:- (d) Galileo Galilei

21. Which of the following is a smallest bacterium?

(a) N.gonorrhoea

(b) M. Tuberculosis

(c) Mycoplasma

(d) Vibrio chlolerae

Answer:- (c) Mycoplasma

22. Palisade arrangement of cells is present in:

(a) M.leprae

(b) C.diphtherae

(c) S.aureus

(d) N.gonorrhoea

Answer:- (b) C.diphtherae

23. The organs of locomotion in bacteria are:

(a) Flagella

(b) Pili

(c) Pseudopods

(d) Both b & c

Answer:- (a) Flagella

24. F.pili or sex pili in bacteria help in:

(a) Binary fission

(c) Locomotion

(b) Respiration

(d) Conjugation

Answer:- (d) Conjugation

25. Capsules are:

(a) Antiphagocytic

(b) Anti-bacteriophagic

(c) Anti toxic

(d) Both a & b

Answer:- (d) Both a & b

26. The backbone of cell wall in prokaryotes is:

(a) Lipopolysaccharide

(b) Polypeptide

(c) Murein

(d) Protein

Answer:- (c) Murein

27. The Glycan portion of peptidoglycan consists of alternating units of:

 (a) NAG & NAM

(b) NAG & ATP

(c) ADP & NAM

(d) Both b & c

Answer:- (a) NAG & NAM

28. The surface antigens present in cell wall of Gram +ve bacteria are:

(a) LPS

(b) Teichoic acids

(c) Lipoproteins

(d) Glycoproteins

Answer:- (b) Teichoic acids

29. Outer membrane is present in cell wall of:

(a) Gram +ve bacteria

(b) Both Gram -ve & Gram +ve bacteria

(c) Only Gram +ve bacteria

(d) Only Gram -ve bacteria

Answer:- (d) Only Gram -ve bacteria

30. The endotoxin present in cell wall of Gram -ve bacteria is:

(a) Polypeptide

(b) Mucopolysaccharide

(c) Lipopolysaccharide

(d) Glycolipid

Answer:- (c) Lipopolysaccharide

31. Membrane invagination in Gram +ve bacteria is called:

(a) Golgi bodies

(b) Ribosomes

(c) ER

(d) Mesosomes

Answer:- (d) Mesosomes

32. The alternative name of nucleoid is:

(a) Nuclear equivalent

(b) Bacterial chromosome

(c) Chromatin body

(d) All the above

Answer:- (d) All the above

33. The DNA present in bacteria is:

(a) Single & circular

(b) Single & linear

(c) Double & circular

(d) Double & linear

Answer:- (a) Single & circular

34. The extrachromosomal piece of DNA in bacteria is:

(a) Mesosome

(b) Plasmid

(c) Plastid

(d) Nucleoid

Answer:- (b) Plasmid

35. Endospores are rich in:

(a) DPA + Ca

(b) DPA + Fe

(c) DPA + Mg

(d) DPA + Cu

Answer:- (a) DPA + Ca

36. A culture by which no fresh nutrients are added & the end products accumulate without their removal is;

(a) Continuous culture

(b) Enhanced culture

(c) Stock culture

(d) Batch culture

Answer:- (d) Batch culture

37. The phase of maximum multiplication resulting in increased population of bacteria with reduced generation time is:

(a) Lag phase

(b) Decline phase

(c) Log phase

(d) Stationary phase

Answer:- (c) Log phase

38. In stationary growth phase the growth rate is:

(a) Equal to death rate

(b) Exceeds death rate

(c) Lower than death rate

(d) None of the above

Answer:- (a) Equal to death rate

39. Bacteria growing best in an atmosphere of 5-10% Co2 are called:

(a) Capnophilic

(b) Mesophilic

(c) Aerophilic

(d) Anaerobic

Answer:- (a) Capnophilic

40. The bacteria which prefer low temperature (0-22ºC) for growth are known as:

(a) Thermophilic

(b) Thermodeuric

(c) Mesophilic

(d) Psychophilic

Answer:- (d) Psychophilic

41. The bacteria which are pathogenic to man & animals are mostly:

(a) Psychrophilic

(b) Thermophilic

(c) Mesophilic

(d) Both b & c

Answer:- (c) Mesophilic

42. Blood agar medium is:

(a) Selective medium

(b) Differential medial

(c) Selective differential medium

(d) All the above

Answer:- (d) All the above

43. Which of the following is not a selective medium?

(a) Nutrient agar medium

(b) Lowenstein Jensen medium

(c) Blood agar medium

(d) Thayer Martin medium

Answer:- (a) Nutrient agar medium

44. Selenite F broth is:

(a) Differential medium

(b) Selective medium

(c) Both a & b

(d) Enrichment medium

Answer:- (d) Enrichment medium

45. VenkataRaman Rama Krishna medium is:

(a) Selective medium

(b) Transport medium

 (c) Differential medium

(d) None of the above

Answer:- (b) Transport medium

46. Which of the following methods are used for obtaining pure culture of microorganisms?

(a) Streak plate method.

(b) Pour plate method

 (c) Spread plate method

 (d) All the above

Answer:- (d) All the above

47. Which of the following methods of isolation of pure cultures was developed by Loeffler & Gaffkey?

(a) Streak plate method

(b) Serial dilution method

(c) Pour plate method

(d) Spread plate method

Answer:- (a) Streak plate method

48. The method of streaking in which there is no overlapping of streaking zones and the loop is sterilized only once in the beginning and at the end is:

(a) Discontinuous method

(b) Continuous method

(c) Consistent method

(d) None of the above

Answer:- (b) Continuous method

49. Streaking method in which streaking is discontinuous & the loop is sterilized between streaking:

(a) Inconsistent method

(b) Continuous method

(c) Discontinuous method

(d) None of the above

Answer:- (c) Discontinuous method

50. Roll tube method of isolation is mostly used for isolation of:

(a) Canophiles

(b) Obligate anaerobes

(c) Aerobes

(d) Obligate aerobes

Answer:- (b) Obligate anaerobes

51. Stab culture is also known as:

(a) Stick cultures

(b) Pick cultures

(c) Slant cultures

(d) Both b & c

Answer:- (a) Stick cultures

52. The method of preservation of culture in which culture is rapidly frozen at a very low temperature -70 °C and then dehydrated by vacuum is:

(a) Paraffination

(b) Cryopreservation

(c) Micromanipulation

(d) Lyophilization

Answer:- (d) Lyophilization

53. Cryopreservation is:

(a) Storage in liquid nitrogen

(b) Storage in nitrogen gas

(c) Storage in liquid hydrogen

(d) Storage in paraffin

Answer:- (a) Storage in liquid nitrogen

54. Which of the following is used as stabilizing agent in cryopreservation?

(a) Glycerol

(b) Dimethyl sulphoxide (DMSO).

(c) Both a & b

(d) Phenol

Answer:- (c) Both a & b

55. Which of the following method based on hydrogen generation in jar is most reliable and widely used for anaerobic culture?

(a) Candle jar method

(b) McIntosh & Filde’s anaerobic

 (c) Gas pack

(d) Pyrogallol method

Answer:- (b) McIntosh & Filde’s anaerobic

56. One of the most common methods of producing an anaerobic environment for microbes by introducing a reducing agent sodium thioglycollate into the liquid medium is:

(a) Candle jar method

(b) McIntosh & Filde’s anaerobic jar

(c) Thioglycollate broth method

 (d) Pyrogallol method

Answer:- (c) Thioglycollate broth method

57. Total count indicating the total number of microbial cell in any given suspension can be determined by which of the following methods?  

(a) Breed method

(b) Counting chamber method

(c) Proportional count method

(d) All the above

Answer:- (d) All the above

58. An electronic instrument can be used for the direct enumeration of cells in a suspension:

(a) Coulter counter

(b) Weils counter

(c) Quebec colony counter

(d) None of the above

Answer:- (a) Coulter counter

59. Type of microscopy useful for the examination of unstained micro-organisms suspended in fluid is:

(a)Electron microscope

(b) Fluorescent microscope

(c) Dark-field microscope

(d) Compound microscope

Answer:- (c) Dark-field microscope

60. Electron microscope was first developed by:

(a) Knoll & Ruska

(b) Glalio Glalie

(c) Louis Pasteur

(d) Antony van Leunhoek

Answer:- (a) Knoll & Ruska

61. A microscope which utilizes a beam of accelerated electrons as a source of illumination for observing objects at a greater magnification is:

(a) Electron microscope

(b) Fluorescent microscope

(c) Dark-field microscope

(d) Compound microscope

Answer:- (a) Electron microscope

62. An electron microscope which forms images using electrons that are transmitted through a specimen:

(a) Transmission Electron microscope

(b) Fluorescent microscope

(c) Dark-field microscope

(d) Scanning electron microscope

Answer:- (a) Transmission Electron microscope

63. An electron microscope that utilize electrons that have bounced off the surface of the specimen:

(a) Transmission Electron microscope

(b) Fluorescent microscope

(c) Dark-field microscope

(d) Scanning electron microscope

Answer:- (d) Scanning electron microscope

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