MCQ/Multiple choice question unit-2 3rd semester (Microbiology) B.Pharmacy

MCQ of unit 2 of Microbiology 3rd semester B.Pharmacy

1. The stains which differentiate two groups of bacteria in a mixture are known as:

  • (a) Negative stain
  • (b) Simple stain
  • (c) Differential stain
  • (d) Indirect stain

Answer:- (c) Differential stain

2. The purpose of simple staining is to demonstrate:

  • (a) Cell size
  • (b) Cell shape
  • (c) Arrangement of bacterial cells
  • (d) All the above

Answer:- (d) All the above

3. Mordant used in Gram staining is:

  • (a) Lugol’s iodine
  • (b) Acetone
  • (c) Peptone
  • (d) Crystal violet

Answer:- (a) Lugol’s iodine

4. Counterstain used in Gram staining is:

  • (a) Lugol’s iodine
  • (b) Safranin
  • (c) Peptone
  • (d) Crystal violet

Answer:- (b) Safranin

5. Acid-fast staining is also known as:

  • (a) Negative staining
  • (b) AFB staining
  • (c) Ziehl-Neelsen staining
  • (d) Both b & c

Answer:- (d) Both b & c

6. IMVIC test is used to differentiate the gram negative enteric bacteria belonging to family:

  • (a) Enterobacteriaceae
  • (b) Chlamydaicae
  • (c) Mycobacteriaceae
  •  (d) None of the above

Answer:- (a) Enterobacteriaceae

7. Citrate utilization test is usually performed on:

  •  (a) Chocolate agar
  • (b) Simmon’s citrate agar
  • (c) Nutrient agar
  • (d) MacConkey agar

Answer:- (b) Simmon’s citrate agar

8. The process of killing microorganisms including spores is:

  • (a) Disinfection
  • (b) Sanitization
  • (c) Sterilization
  • (d) Filtration

Answer:- (c) Sterilization

9. Sanitization is mostly used in:

  • (a) Hospitals
  • (b) Research laboratories
  •  (c) Food processing & catering units
  •  (d) All the above

Answer:- (c) Food processing & catering units

10. Paraffin oil can be sterilized by:

  • (a) Hot air oven
  • (b) Autoclaving
  • (c) Filtration
  • (d) Disinfection

Answer:- (a) Hot air oven

11. The flash & holder methods are used in the process of:

  • (a) Immunization
  • (b) Disinfection
  • (c) Pasteurization
  • (d) Both a & b

Answer:- (c) Pasteurization

12. The process of sterilization by intermittent heating for 3 successive days is known as:

  • (a) Inspissation
  • (b) Tyndallization
  • (c) Pasteurization
  • (d) Incineration

Answer:- (b) Tyndallization

13. Best autoclaving is achieved at:

  • (a) 121°C for 15 minutes
  • (b) 120°C for 1 hour
  • (c) 110°C for 2 hours
  • (d) 100°C for 3 hours

Answer:- (a) 121°C for 15 minutes

14. Burning to ashes is referred to as:

  • (a) Inertization
  • (b) Sanitization
  • (c) Incineration
  • (d) Disinfection

Answer:- (c) Incineration

15. By inspissation repeated for three consecutive days, some degree of sterilization is achieved which is known as:

  • (a) Fractional sterilization
  • (b) Sanitization
  • (c) Incineration
  • (d) Disinfection

Answer:- (a) Fractional sterilization

16. Filter which is disposable of single-use discs made up of asbestos (magnesium trisilicate) is:

  • (a) Sintered glass filter
  • (b) Seitz filter
  • (c) Membrane filter
  • (d) Berkefield filter

Answer:- (b) Seitz filter

17. Sintered glass filter includes:

  • (a) Fritted glass filter
  • (b) Morton filters
  • (c) Both a & b
  • (d) Berkefield filter

Answer:-  (c) Both a & b

18. Filters of different grades of porosity and used widely for purification of water for industrial and drinking purposes are:

  • (a) Sintered glass filter
  • (b) Seitz filter
  • (c) Membrane filter
  • (d) Berkefield filter

Answer:- (d) Berkefield filter

19. Sterilization by ionizing radiation is also known as:

  • (a) Cold sterilization
  • (b) Thermosterilization
  • (c) Electrosterilization
  • (d) None of the above

Answer:- (a) Cold sterilization

20. Which of the following chemicals in gaseous form can be used disinfection and sterilization of enclosed area such as operation theatres, hospital rooms, aseptic area and microbiology laboratories?

  • (a) Glutaraldehdyde
  • (b) Formaldehyde
  • (c) Ethylene oxide
  • (d) None of the above

Answer:- (b) Formaldehyde

21. Sterilant gas used for sterilization of heat sensitive plastic disposable items is:

  • (a) Glutaraldehdyde
  • (b) Formaldehyde
  • (c) Ethylene oxide
  •  (d) None of the above

Answer:- (c) Ethylene oxide

22. Which of the following are chemical indicators of sterilization?

  • (a) Browne’s tube
  • (b) Witness tube
  • (c) Royce sachet
  • (d) All the above

Answer:- (d) All the above

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