Chromatography Retention Factor (Rf) Calculator Retention Factor (Rf) Calculator Distance traveled by solute (spot) cmmminches Distance traveled by solvent front cmmminches Calculate Rf Reset Calculation Result Retention Factor (Rf) Step-by-Step Breakdown Chromatogram Diagram Export Result as JPG Export Result as PDF What is the Retention Factor (Rf)? The Retention Factor (Rf) is a fundamental quantity … Read more
Concentration from Absorbance Calculator Concentration from Absorbance Calculator Absorbance (A) (unitless) Molar Extinction Coefficient (ε) M⁻¹cm⁻¹ Path Length (l) cm Calculate Concentration Reset Calculation Result Concentration (c) Step-by-Step Breakdown The Beer-Lambert Law The Beer-Lambert Law (also known as Beer’s Law) is a fundamental principle in optics and chemistry that relates the attenuation of light to … Read more
Codon Usage Calculator / Translator Codon Usage Calculator / Translator DNA Input (A, T, C, G) RNA Input (A, U, C, G) Nucleic Acid Sequence Clean Input Reset Translate & Analyze Analysis Results Translated Protein Sequence Codon Usage Codon AA Count Freq (%) Codon AA Count Freq (%) Synonymous Codon Usage Chart Export Result as … Read more
Sequence Molecular Mass Calculator Sequence Molecular Mass Calculator Peptide Oligonucleotide Peptide Sequence (One-Letter Codes) Clean Input Type: DNA RNA 5′ Triphosphate End Calculate Mass Reset Calculation Results Calculated Molecular Mass 0 Da Sequence Composition Calculating Biopolymer Molecular Mass The molecular mass (or molecular weight) of a biopolymer like a protein or nucleic acid is the … Read more
DNA/RNA Reverse Complement Generator DNA/RNA Reverse Complement Generator DNA (A, T, C, G) RNA (A, U, C, G) Nucleic Acid Sequence Clean Input Reset Generate Generated Sequences Complement Sequence (3′ to 5′): Reverse Complement Sequence (5′ to 3′): Copy What is a Reverse Complement? In molecular biology, the reverse complement of a nucleic acid sequence … Read more
GC Content Calculator GC Content Calculator DNA Sequence (A, T, C, G) Clean Input Reset Calculate GC Content Analysis Results GC Content 0.00% Base Counts A Count0 T Count0 C Count0 G Count0 GC Total0 Total Bases0 Base Composition Chart Export Result as JPG Export Result as PDF What is GC Content? GC content (or … Read more
Extinction Coefficient Calculator (protein/DNA absorbance at 280 nm) Protein ssDNA (280nm) Protein Sequence (One-Letter Codes) Clean Input Molar Concentration (c) mol/L (M) Path Length (l) cm Calculate Reset Calculation Results Extinction Coefficient (ε₂₈₀) 0 M⁻¹cm⁻¹ Absorbance at 280nm (A₂₈₀) 0 Calculation Breakdown Extinction Coefficient & Beer-Lambert Law The Molar Extinction Coefficient (ε), also known as … Read more
Isoelectric Point (pI) Calculator Isoelectric Point (pI) Calculator Single Amino Acid Peptide Sequence Select Amino Acid Peptide Sequence (One-Letter Codes) Clean Input Calculate pI Reset Calculation Result Isoelectric Point (pI) Titration Curve (Net Charge vs. pH) Export Result as JPG Export Result as PDF What is the Isoelectric Point (pI)? The Isoelectric Point (pI) is … Read more
Amino Acid Composition Analyzer Amino Acid Composition Analyzer Protein Sequence (One-Letter Codes) Clean Input Reset Analyze Sequence Analysis Results Total Amino Acids 0 Unknown Characters 0 Molecular Weight 0 Da Composition Breakdown Name Code Count Percent (%) Percentage Composition Chart Export Result as JPG Export Result as PDF What is Amino Acid Composition Analysis? Amino … Read more
Osmolarity & Osmolality Calculator Osmolarity & Osmolality Calculator Calculate Osmolarity Calculate Osmolality Solutes + Add Solute Total Volume of Solution Liters (L)Milliliters (mL) Total Mass of Solvent Kilograms (kg)Grams (g) Calculate Reset Calculation Result Step-by-Step Breakdown Osmolarity vs. Osmolality Osmolarity and Osmolality are both measures of the total concentration of solute particles in a solution, … Read more
