Hybridoma technology – production and applications of monoclonal antibodies MCQs With Answer
Introduction Hybridoma technology is a foundational method for the production of monoclonal antibodies (mAbs), combining antibody-producing B cells with immortal myeloma cells to create hybridomas. This process—involving fusion agents like PEG, HAT selection, limiting dilution cloning, and screening by ELISA or flow cytometry—yields specific, high-affinity IgG antibodies for diagnostics, therapeutics, and research. Key steps include … Read more
