MULTIPLE CHOICE QUESTIONS / MCQ Unit-4(Aseptic Area and Microbial Control, Microbiological Assay), Microbiology, 3rd Semester, B.Pharmacy
1. Counters especially useful in determining the number of particle/microbes count per cube feet to classify the cleanliness of a particular room or area are:
- a) Electronic air particle counters
- b) Settle plates
- c) Electrochemical counters
- d) None of the above
Answer:- a) Electronic air particle counters
2. Settle plate method is also known as:
- a) Spread plate method
- b) Floatation method
- c) Pour plate method
- d) Sedimentation method
Answer:- d) Sedimentation method
3. Limit for microbial contamination of an operating clean room Grade C for viable microorganisms/m’ of air is:
- a) 100
- b) 200
- c) 500
- d) 1000
Answer:- a) 100
4. Air velocity of a HEPA filter is:
- a) 100+10 m/sec
- b) 1000+ 20 m/sec
- c) 90+ 20 feet/min
- d) 120 10 feet/min.
Answer:- c) 90+ 20 feet/min
5. DOP test is used for validation of:
- a) Membrane filter
- b) HEPA filter
- c) Aseptic room
- d) Autoclave
Answer:- HEPA filter
6. Efficiency of HEPA filter is:
- a) 99.97
- b) 88.87
- c) 90.97
- d) 98.97
Answer:- a) 99.97
7. The velocity of HEPA filtered air is measured by:
- a) Galvanometer
- b) Speedometer
- c) Velometer
- d) Turbidometer
Answer:- c) Velometer
8. Surface sample method using sterile cotton swab tip to sample locations is:
- a) Rodac plate test
- b) Swab rinse test
- c) Biotest
- d) None of the above
Answer:- b) Swab rinse test
9. Test organism used for microbiological assay of Neomycin is:
- a) Bacillus pumilus
- b) Staph.aureus
- c) Staph.epidermidis
- d) Saccharomyces cerevisiae
Answer:- c) Staph.epidermidis
10. Test organism used for microbiological assay of Vitamin B₁2 is:
- a) Lactobacillus leichamannii
- b) Lactobacillus casei
- c) Lactobacillus viridescens
- d) Lactobacillus plantarum
Answer:- a) Lactobacillus leichamannii
11. In broth dilution test, the final inoculum used for determination of MIC of antimicrobial compound is:
- a) 10¹ CFU/ml
- b) 105 CFU/ml
- c) 10 CFU/ml
- d) 1010 CFU/ml
Answer:- b) 105 CFU/ml
12. Staphylococcus aureus is used for IP assay of:
- a) Bleomycin
- b) Carbenicillin
- c) Doxycycline
- d) KanamycinKanamycin
Answer:- c) Doxycycline
13. In microbial assay of bacitracin IP, the test organism used is:
- a) Micrococcus luteus
- b) Pasteurella pestis
- c) Bacillus subtilis
- d) Staphylococcus aureus
Answer:- a) Micrococcus luteus
14. In microbial assay of Nystatin, the test organism used is:
- a) Saccharomyces cerevisiae
- b) Bacillus subtilis
- c) E.coli
- d) Bacillus pumilus
Answer:- a) Saccharomyces cerevisiae
15. For assay of amino acid alanine, the test organism used is:
- a) Staph.aureus citrovorum
- b) Bacillus subtilis
- c) Strep faecalis
- d) Leuconostoc citrovorum
Answer:- d) Leuconostoc citrovorum
MULTIPLE CHOICE QUESTIONS / MCQ Unit-5(Microbial Spoilage, Preservation of pharmaceutical Products, Growth of Animal Cell in Culture) Microbiology, 3rd Semester, B.Pharmacy
1. The test designed for estimation of number of viable microorganisms present & for detecting the presence of species microbial species in pharmaceutical substances is:
- a) Microbial limit test
- b) Litmus test
- c) Microbial load test
- d) None of the above
Answer:- a) Microbial limit test
2. Microbial limit of aluminium hydroxide gel for total viable count is less than:
- a)10/ml
- b) 100/ml
- c) 500/ml
- d) 1000/ml
Answer:- b) 100/ml
3. Total aerobic microbial count present in test substances is determined as per I.P. by following methods except:
- a) Membrane filtration
- b) Total plate count
- c) Most probable number
- d) Turbidimetric method
Answer:- d) Turbidimetric method
4. Glycerol may be used as preservative upto a percentage of:
- a) 15
- b) 50
- c) 10
- d) 1
Answer:- b) 50
5. All the following test microorganisms are used for preservative efficacy test except:
- a) Staphylococcus aureus
- b) Bacillus subtilis
- c) Aspergillus niger
- d) Candida albicans
Answer:- b) Bacillus subtilis
6. Which of the following mould is used for preventative efficacy test?
- a) Aspergillus brasiliensis
- b) Bacillus subtilis
- c) Aspergillus niger
- d) Candida albicans
Answer:- a) Aspergillus brasiliensis
7. When a finite cell line undergoes transformation and acquires the ability to divide indefinitely. it becomes:
- a) Continuous cell line
- c) Infinite cell line
- b) Discontinuous cell line
- d) Both b & C
Answer:- a) Continuous cell line
