Multiple Choice Question / MCQ Microbiology unit-4&5 3rd Semester

MULTIPLE CHOICE QUESTIONS / MCQ Unit-4(Aseptic Area and Microbial Control, Microbiological Assay), Microbiology, 3rd Semester, B.Pharmacy

1. Counters especially useful in determining the number of particle/microbes count per cube feet to classify the cleanliness of a particular room or area are:

  • a) Electronic air particle counters
  • b) Settle plates
  • c) Electrochemical counters
  • d) None of the above

Answer:- a) Electronic air particle counters

2. Settle plate method is also known as:

  • a) Spread plate method
  • b) Floatation method
  • c) Pour plate method
  • d) Sedimentation method

Answer:- d) Sedimentation method

3. Limit for microbial contamination of an operating clean room Grade C for viable microorganisms/m’ of air is:

  • a) 100
  • b) 200
  • c) 500
  • d) 1000

Answer:- a) 100

4. Air velocity of a HEPA filter is:

  • a) 100+10 m/sec
  • b) 1000+ 20 m/sec
  • c) 90+ 20 feet/min
  • d) 120 10 feet/min.

Answer:- c) 90+ 20 feet/min

5. DOP test is used for validation of:

  • a) Membrane filter
  • b) HEPA filter
  • c) Aseptic room
  • d) Autoclave

Answer:- HEPA filter

6. Efficiency of HEPA filter is:

  • a) 99.97
  • b) 88.87
  • c) 90.97
  • d) 98.97

Answer:- a) 99.97

7. The velocity of HEPA filtered air is measured by:

  • a) Galvanometer
  • b) Speedometer
  • c) Velometer
  • d) Turbidometer

Answer:- c) Velometer

8. Surface sample method using sterile cotton swab tip to sample locations is:

  • a) Rodac plate test
  • b) Swab rinse test
  • c) Biotest
  • d) None of the above

Answer:- b) Swab rinse test

9. Test organism used for microbiological assay of Neomycin is:

  • a) Bacillus pumilus
  • b) Staph.aureus
  • c) Staph.epidermidis
  • d) Saccharomyces cerevisiae

Answer:- c) Staph.epidermidis

10. Test organism used for microbiological assay of Vitamin B₁2 is:

  • a) Lactobacillus leichamannii
  • b) Lactobacillus casei
  • c) Lactobacillus viridescens
  • d) Lactobacillus plantarum

Answer:- a) Lactobacillus leichamannii

11. In broth dilution test, the final inoculum used for determination of MIC of antimicrobial compound is:

  • a)  10¹ CFU/ml
  • b)  105 CFU/ml
  • c)  10 CFU/ml
  • d) 1010 CFU/ml

Answer:- b)  105 CFU/ml

12. Staphylococcus aureus is used for IP assay of:

  • a) Bleomycin
  • b) Carbenicillin
  • c) Doxycycline
  • d) KanamycinKanamycin

Answer:- c) Doxycycline

13. In microbial assay of bacitracin IP, the test organism used is:

  • a) Micrococcus luteus
  • b) Pasteurella pestis
  • c) Bacillus subtilis
  • d) Staphylococcus aureus

Answer:- a) Micrococcus luteus

14. In microbial assay of Nystatin, the test organism used is:

  • a) Saccharomyces cerevisiae
  • b) Bacillus subtilis
  • c) E.coli
  • d) Bacillus pumilus

Answer:- a) Saccharomyces cerevisiae

15. For assay of amino acid alanine, the test organism used is:

  • a) Staph.aureus citrovorum
  • b) Bacillus subtilis
  • c) Strep faecalis
  • d) Leuconostoc citrovorum

Answer:- d) Leuconostoc citrovorum

MULTIPLE CHOICE QUESTIONS / MCQ Unit-5(Microbial Spoilage, Preservation of pharmaceutical Products, Growth of Animal Cell in Culture) Microbiology, 3rd Semester, B.Pharmacy

1. The test designed for estimation of number of viable microorganisms present & for detecting the presence of species microbial species in pharmaceutical substances is:

  • a) Microbial limit test
  • b) Litmus test
  • c) Microbial load test
  • d) None of the above

Answer:- a) Microbial limit test

2. Microbial limit of aluminium hydroxide gel for total viable count is less than:

  • a)10/ml
  • b) 100/ml
  • c) 500/ml
  • d) 1000/ml

Answer:- b) 100/ml

3. Total aerobic microbial count present in test substances is determined as per I.P. by following methods except:

  • a) Membrane filtration
  • b) Total plate count
  • c) Most probable number
  • d) Turbidimetric method

Answer:- d) Turbidimetric method

4. Glycerol may be used as preservative upto a percentage of:

  • a) 15
  • b) 50
  • c) 10
  • d) 1

Answer:- b) 50

5. All the following test microorganisms are used for preservative efficacy test except:

  • a) Staphylococcus aureus
  • b) Bacillus subtilis
  • c) Aspergillus niger
  • d) Candida albicans

Answer:- b) Bacillus subtilis

6. Which of the following mould is used for preventative efficacy test?

  •  a) Aspergillus brasiliensis
  • b) Bacillus subtilis
  • c) Aspergillus niger
  • d) Candida albicans

Answer:- a) Aspergillus brasiliensis

7. When a finite cell line undergoes transformation and acquires the ability to divide indefinitely. it becomes:

  • a) Continuous cell line
  • c) Infinite cell line
  • b) Discontinuous cell line
  • d) Both b & C

Answer:- a) Continuous cell line

Leave a Comment