Internet resources in bioinformatics MCQs With Answer

Introduction: This quiz set on Internet resources in bioinformatics is tailored for M.Pharm students aiming to strengthen practical knowledge of online databases and computational tools used in pharmaceutics and molecular research. It covers nucleotide and protein repositories, structural databases, pathway and enzyme resources, literature archives, sequence-analysis tools, common file formats and interpretation of search metrics. Each multiple-choice question targets concepts you will apply in drug discovery, pharmacogenomics, and data mining tasks—such as choosing the right database for sequence retrieval, understanding accession prefixes, interpreting BLAST results, and selecting pathway or variant resources. Answers are provided to support quick self-assessment and targeted revision.

Q1. Which database at NCBI is the primary public repository for nucleotide sequences?

• GenBank
• EMBL-EBI
• DDBJ
• RefSeq

Correct Answer: GenBank

Q2. Which section of UniProt contains manually reviewed, high-quality protein annotations?

• TrEMBL
• Swiss-Prot
• PDB
• KEGG

Correct Answer: Swiss-Prot

Q3. Which online tool is commonly used for detecting sequence similarity against NCBI databases?

• BLAST
• Clustal Omega
• HMMER
• MAFFT

Correct Answer: BLAST

Q4. What is the standard file format for macromolecular 3D coordinates widely used in structural biology?

• FASTA
• PDB
• GFF
• GenBank

Correct Answer: PDB

Q5. In BLAST output, what does the E-value indicate?

• Expected number of alignments with a similar score due to chance
• Percent identity between sequences
• Raw alignment score only
• Query coverage percentage

Correct Answer: Expected number of alignments with a similar score due to chance

Q6. Which online resource focuses on drugs, drug actions and drug–target relationships useful for pharmacology?

• BRENDA
• DrugBank
• UniProt
• PDB

Correct Answer: DrugBank

Q7. Which database integrates genomic information into metabolic and signaling pathway maps?

• KEGG
• PubMed
• GEO
• SRA

Correct Answer: KEGG

Q8. Which prefix denotes a RefSeq curated mRNA record at NCBI?

• NM_
• XM_
• NP_
• XP_

Correct Answer: NM_

Q9. Which repository stores raw high-throughput sequencing reads submitted to NCBI?

• SRA
• GEO
• PDB
• UniProt

Correct Answer: SRA

Q10. Where are processed gene expression data from microarrays and RNA‑seq commonly deposited?

• GEO
• SRA
• PubMed
• KEGG

Correct Answer: GEO

Q11. BLASTn and BLASTp are specialized for which pair of sequence types?

• Nucleotide queries vs protein queries respectively
• Protein queries vs nucleotide queries respectively
• Both are identical and accept any sequence
• BLASTn translates nucleotide to protein before searching

Correct Answer: Nucleotide queries vs protein queries respectively

Q12. Which database is specifically designed to catalog protein families and conserved domains?

• Pfam
• PubChem
• DDBJ
• Ensembl

Correct Answer: Pfam

Q13. Which web tool is commonly used for generating multiple sequence alignments for phylogeny and conservation studies?

• Clustal Omega
• BLAST
• FASTA
• PubMed

Correct Answer: Clustal Omega

Q14. Which database should you consult for experimentally determined 3D structures of proteins and nucleic acids?

• PDB
• KEGG
• DrugBank
• UniProt

Correct Answer: PDB

Q15. Which resource provides standardized functional annotations across genes and proteins using a controlled vocabulary?

• Gene Ontology (GO)
• Ensembl
• PubMed
• Swiss-Prot

Correct Answer: Gene Ontology (GO)

Q16. Which search tool uses profile Hidden Markov Models for sensitive domain and family detection?

• HMMER
• BLAST
• ClustalW
• MAFFT

Correct Answer: HMMER

Q17. Which database specializes in enzyme information including kinetics, substrates and inhibitors?

• BRENDA
• Pfam
• PDB
• GEO

Correct Answer: BRENDA

Q18. Which file format is commonly used to describe genomic features (locations of genes, exons, CDS) for genome browsers?

• GFF
• FASTA
• GenBank
• PDB

Correct Answer: GFF

Q19. Which online resource is the primary indexed repository for biomedical literature and abstracts?

• PubMed
• PubChem
• UniProt
• SRA

Correct Answer: PubMed

Q20. Which database aggregates clinical interpretations of genetic variants relevant to human health?

• ClinVar
• RefSeq
• GenBank
• GEO

Correct Answer: ClinVar

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