Genetic Engineering Applications | Biotechnology | GPAT Mock Test

1. What is the primary function of Taq polymerase in a Polymerase Chain Reaction (PCR)?

2. Which step in PCR involves heating to approximately 94-98°C to separate the double-stranded DNA?

3. The three main steps in a PCR cycle are, in the correct order:

4. Reverse Transcriptase PCR (RT-PCR) is specifically used to amplify:

5. What component of a PCR reaction provides the specific starting point for DNA synthesis?

6. Gene therapy primarily aims to:

7. Which type of gene therapy involves modifying germ cells (sperm or eggs), leading to heritable changes?

8. The most commonly used vectors for delivering genes in human gene therapy are:

9. ‘Ex vivo’ gene therapy is characterized by:

10. Quantitative PCR (qPCR) differs from standard PCR by:

11. In PCR, what is the purpose of deoxynucleoside triphosphates (dNTPs)?

12. A major challenge associated with using viral vectors in gene therapy is:

13. Which of the following is an example of a non-viral drug delivery system for gene therapy?

14. What is the role of a “promoter” sequence in a gene therapy vector?

15. SCID (Severe Combined Immunodeficiency) was one of the first diseases successfully treated using which technique?

16. The primary concern with using retroviruses as vectors for gene therapy is the risk of:

17. Which of the following is NOT a required component of a typical PCR master mix?

18. ‘In vivo’ gene therapy refers to the process where:

19. Hot-start PCR is a technique used primarily to:

20. What is a major application of PCR in forensic science?

21. Cationic liposomes are effective for gene delivery because they:

22. The CRISPR-Cas9 system is a revolutionary tool in gene therapy primarily for its ability to:

23. The temperature for the annealing step in PCR is typically:

24. What type of vector, known for infecting both dividing and non-dividing cells, is a modified version of the HIV virus?

25. The term “thermal cycler” refers to the instrument used for: