Extraction & Isolation Techniques | Pharmacognosy | GPAT Mock Test

Welcome to this specialized mock test on Extraction and Isolation Techniques, a crucial topic in Pharmacognosy for the GPAT examination. This quiz is designed to challenge your understanding of the fundamental principles and practical applications used to separate and identify phytopharmaceuticals. You will be tested on general methods for extracting alkaloids, glycosides, flavonoids, volatile oils, and more. The quiz also covers the application of essential chromatographic techniques like Column, Paper, and Thin Layer Chromatography (TLC) in phytochemistry. This practice test contains 25 multiple-choice questions to help you assess your preparation, identify areas for improvement, and build confidence. After submitting your answers, you can download all questions with the correct answers in a PDF format for future revision. Good luck!

1. The Stas-Otto process is a classical method primarily used for the extraction and purification of which class of phytoconstituents?

2. In chromatography, the term ‘elution’ refers to:

3. The Shinoda test, which produces a magenta or pink color, is a specific identification test for:

4. The Rf value in Thin Layer Chromatography (TLC) is calculated by which formula?

5. Which of the following extraction methods is most suitable for obtaining volatile oils from delicate flower petals like jasmine or rose?

6. The ability of saponins to cause hemolysis of red blood cells is a key characteristic used for their identification. This property is due to their:

7. In paper chromatography, what serves as the stationary phase?

8. Soxhlet apparatus provides a continuous extraction method that is most efficient for extracting which type of compounds from a solid matrix?

9. In the general extraction of alkaloids, the plant material is first treated with an alkali like ammonia to:

10. Dragendorff’s reagent, which gives a reddish-brown precipitate, is used for the detection of:

11. The non-sugar component of a glycoside, which remains after the sugar part is hydrolyzed, is known as:

12. In normal-phase column chromatography, which of the following would be the most suitable adsorbent (stationary phase)?

13. The Froth test, involving vigorous shaking of a drug extract with water, is a preliminary screening test for:

14. Resins are complex amorphous mixtures that are characteristically insoluble in water but soluble in:

15. The Killer-Kiliani test is a specific chemical test for the identification of:

16. Which general extraction method involves placing the crude drug in a stoppered container with a solvent and allowing it to stand at room temperature for a defined period with frequent agitation?

17. In column chromatography, separation of components is based on:

18. The most common method for commercial extraction of volatile oils from hardy plant materials like cloves or cinnamon bark is:

19. A ‘balsam’ is a type of resinous mixture that specifically contains a high proportion of:

20. What is the primary reason for using a non-polar solvent like hexane or petroleum ether for the initial defatting step in the extraction of polar compounds?

21. Mayer’s reagent gives a ________ precipitate with alkaloids.

22. Which of these is a common non-destructive visualizing agent used in TLC for many organic compounds?

23. In the isolation of flavonoids, lead acetate is often used to:

24. Decoction is an extraction process best suited for:

25. In reverse-phase chromatography (e.g., on a C18 column), what is the typical nature of the stationary and mobile phases?