Recombinant DNA Technology MCQ Quiz | Microbial Genetics

1. Which enzyme is responsible for cutting DNA at specific palindromic sequences?

2. In blue-white screening, the blue color is produced by the enzymatic breakdown of X-gal. This breakdown is mediated by which enzyme?

3. Which of the following is NOT an essential feature of a cloning vector?

4. The technique used to amplify a specific DNA sequence exponentially is:

5. ‘Sticky ends’, generated by certain restriction enzymes, are:

6. A cDNA library is a collection of DNA fragments that represent:

7. In producing human insulin in E. coli, the enzyme used to create cDNA from mature mRNA is:

8. Which vector can carry the largest DNA insert?

9. Gel electrophoresis separates DNA fragments based primarily on their:

10. The role of DNA ligase in recombinant DNA technology is to:

11. A selectable marker, such as an antibiotic resistance gene (e.g., ampR), is used to:

12. The three main steps in a single PCR cycle, in correct order, are:

13. Southern blotting is a technique used for the detection of:

14. The introduction of foreign DNA into a bacterial cell via a viral vector is called:

15. In a pUC18 vector, insertional inactivation of the lacZ gene allows for:

16. Taq polymerase, used in PCR, is a thermostable enzyme isolated from:

17. What is the primary purpose of a ‘probe’ in blotting techniques like Southern or Northern blotting?

18. Cosmids are hybrid vectors derived from:

19. The process of making a bacterial cell competent to take up foreign DNA is often achieved by:

20. Restriction Fragment Length Polymorphism (RFLP) analysis is used in:

21. Which of the following components is NOT required for a standard PCR reaction?

22. Western blotting is used to detect the presence of a specific:

23. The ‘multiple cloning site’ (MCS) or polylinker in a vector is a region that contains:

24. In recombinant DNA technology, the term “vector” refers to:

25. The Agrobacterium tumefaciens Ti plasmid is widely used as a vector for: