Recombinant DNA Technology MCQ Quiz | Microbial Genetics

Welcome to the Recombinant DNA Technology quiz, designed for MBBS students studying Microbial Genetics. This assessment will test your understanding of the fundamental principles and applications of genetic engineering. You will encounter 25 multiple-choice questions covering key topics such as restriction enzymes, cloning vectors, PCR, gene libraries, and blotting techniques. This quiz is an excellent tool for self-evaluation and reinforcing your knowledge of how DNA molecules are manipulated in the lab to create new combinations of genetic material. Upon completion, your score will be displayed, and you will see the correct answers highlighted. For future reference and revision, you can also download a PDF file containing all the questions and their correct answers. Good luck!

1. Which enzyme is responsible for cutting DNA at specific palindromic sequences?

2. In blue-white screening, the blue color is produced by the enzymatic breakdown of X-gal. This breakdown is mediated by which enzyme?

3. Which of the following is NOT an essential feature of a cloning vector?

4. The technique used to amplify a specific DNA sequence exponentially is:

5. ‘Sticky ends’, generated by certain restriction enzymes, are:

6. A cDNA library is a collection of DNA fragments that represent:

7. In producing human insulin in E. coli, the enzyme used to create cDNA from mature mRNA is:

8. Which vector can carry the largest DNA insert?

9. Gel electrophoresis separates DNA fragments based primarily on their:

10. The role of DNA ligase in recombinant DNA technology is to:

11. A selectable marker, such as an antibiotic resistance gene (e.g., ampR), is used to:

12. The three main steps in a single PCR cycle, in correct order, are:

13. Southern blotting is a technique used for the detection of:

14. The introduction of foreign DNA into a bacterial cell via a viral vector is called:

15. In a pUC18 vector, insertional inactivation of the lacZ gene allows for:

16. Taq polymerase, used in PCR, is a thermostable enzyme isolated from:

17. What is the primary purpose of a ‘probe’ in blotting techniques like Southern or Northern blotting?

18. Cosmids are hybrid vectors derived from:

19. The process of making a bacterial cell competent to take up foreign DNA is often achieved by:

20. Restriction Fragment Length Polymorphism (RFLP) analysis is used in:

21. Which of the following components is NOT required for a standard PCR reaction?

22. Western blotting is used to detect the presence of a specific:

23. The ‘multiple cloning site’ (MCS) or polylinker in a vector is a region that contains:

24. In recombinant DNA technology, the term “vector” refers to:

25. The Agrobacterium tumefaciens Ti plasmid is widely used as a vector for: