Glycolysis MCQ Quiz | Carbohydrate Metabolism

1. Where does glycolysis primarily occur in a eukaryotic cell?

2. Which enzyme catalyzes the first irreversible, committed step in glycolysis?

3. Which of the following is a potent allosteric activator of Phosphofructokinase-1 (PFK-1)?

4. Substrate-level phosphorylation in glycolysis involves the direct transfer of a phosphate group to ADP. Which two enzymes catalyze these reactions?

5. What is the net yield of ATP and NADH per molecule of glucose in glycolysis under aerobic conditions?

6. In anaerobic conditions in skeletal muscle, pyruvate is converted to lactate. What is the primary purpose of this reaction?

7. Fluoride is a potent inhibitor of glycolysis. It specifically inhibits which enzyme?

8. Which intermediate of glycolysis is a key precursor for the synthesis of glycerol-3-phosphate, essential for triglyceride synthesis?

9. Glucokinase (Hexokinase IV) differs from other hexokinase isozymes in that it:

10. The conversion of Glucose-6-Phosphate to Fructose-6-Phosphate is an example of which type of reaction?

11. Arsenate poisoning disrupts glycolysis by substituting for inorganic phosphate. This uncouples which reaction, leading to no net ATP synthesis?

12. The “splitting” of Fructose 1,6-bisphosphate into two triose phosphates is catalyzed by which enzyme?

13. The Rapoport-Luebering shunt in erythrocytes bypasses an ATP-generating step to produce which important molecule that modulates hemoglobin’s oxygen affinity?

14. Which of the following is NOT an intermediate of the glycolytic pathway?

15. A deficiency in which glycolytic enzyme is a common cause of hereditary non-spherocytic hemolytic anemia due to insufficient ATP production in RBCs?

16. During the preparatory phase of glycolysis (first 5 steps), how many ATP molecules are consumed per molecule of glucose?

17. Which hormone stimulates glycolysis, particularly in the liver, by promoting the synthesis of glucokinase, PFK-1, and pyruvate kinase?

18. The conversion of 2-Phosphoglycerate to Phosphoenolpyruvate (PEP) is catalyzed by Enolase and involves the removal of which molecule?

19. The high-energy phosphate bond in which molecule drives the final substrate-level phosphorylation step of glycolysis?

20. Fructose metabolism in the liver bypasses the main regulatory step of glycolysis (PFK-1). How does it enter the pathway?

21. Which coenzyme is essential for the reaction catalyzed by Glyceraldehyde-3-phosphate dehydrogenase?

22. The enzyme triose phosphate isomerase catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and:

23. Which of the following statements about anaerobic glycolysis is TRUE?

24. Galactose enters the glycolytic pathway after being converted to:

25. The Warburg effect, often seen in cancer cells, describes a high rate of glycolysis followed by lactic acid fermentation even in the presence of oxygen. This phenomenon is also known as: